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Human CD209 Recombinant Protein (N-Fc) (HV997011)

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Human CD209 Recombinant Protein (N-Fc)

Human CD209 Recombinant Protein (N-Fc)

Human CD209 Recombinant Protein (N-Fc)

Human CD209 Recombinant Protein (N-Fc)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HV997011
Description	Recombinant Human CD209 Protein, N-Fc (HV997011) expressed in Mammalian Cells, spanning Lys62-Ala404. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	Q9NNX6
Protein length	Lys62-Ala404
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	67.20 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CD209, DC-SIGN1, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, CD209 antigen, CLEC4L, DC-SIGN, C-type lectin domain family 4 member L
Background	CD209 antigen is a ~45 kDa protein. Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD209 Protein, N-Fc
Bioactivity

Detects Human CD209 Antibody (2B8) in indirect ELISAs.

References

HV997011-SDS-PAGE-1.jpg

Datasheet

Human CD209 Recombinant Protein (N-Fc).pdf

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Formula

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Human CD209 Recombinant Protein (N-Fc)