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Recombinant Human CTSG Protein, N-GST (HY284012)

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Recombinant Human CTSG Protein, N-GST

Recombinant Human CTSG Protein, N-GST

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Datasheet

Overview

Catalog No.	HY284012
Description	Recombinant Human CTSG Protein, N-GST (HY284012) expressed in E. coli, spanning Ile21-Leu255. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P08311
Protein length	Ile21-Leu255
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	53.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cathepsin G, CG, CTSG
Background	Cathepsin G (CTSG) is a ~28 kDa protein. Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor. 1. Thorpe, M. et al. (2018) PloS one 13, e0195077. PMID: 29652924 2. Avril, LE. et al. (1994) FEBS letters 345, 81-6. PMID: 8194606 3. Bangalore, N. et al. (1990) The Journal of biological chemistry 265, 13584-8. PMID: 2116408 4. Alford, CE. et al. (1990) Journal of general microbiology 136, 997-100. PMID: 2117044 5. Sambrano, GR. et al. (2000) The Journal of biological chemistry 275, 6819-23. PMID: 10702240 6. Selak, MA. et al. (1988) The Biochemical journal 251, 293-9. PMID: 3390156 7. Hase-Yamazaki, T. et al. (1995) Cellular immunology 160, 24-32. PMID: 7842483 8. Yamazaki, T. et al. (1997) Journal of leukocyte biology 61, 73-9. PMID: 9000539
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CTSG Protein, N-GST

References

HY284012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human CTSG Protein, N-GST.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Recombinant Human CTSG Protein, N-GST