Anti-CTSG Polyclonal Antibody (HY284014)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

HY284014

Description

Anti-CTSG Polyclonal Antibody (HY284014) is a rabbit polyclonal antibody detecting CTSG in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CTSG (Ile21-Leu255).

Target

Cathepsin G, CG, CTSG

Purification

Purified by antigen affinity column.

Accession

P08311

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cathepsin G (CTSG) is a ~28 kDa protein. Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor.

1. Thorpe, M. et al. (2018) PloS one 13, e0195077. PMID: 29652924

2. Avril, LE. et al. (1994) FEBS letters 345, 81-6. PMID: 8194606

3. Bangalore, N. et al. (1990) The Journal of biological chemistry 265, 13584-8. PMID: 2116408

4. Alford, CE. et al. (1990) Journal of general microbiology 136, 997-100. PMID: 2117044

5. Sambrano, GR. et al. (2000) The Journal of biological chemistry 275, 6819-23. PMID: 10702240

6. Selak, MA. et al. (1988) The Biochemical journal 251, 293-9. PMID: 3390156

7. Hase-Yamazaki, T. et al. (1995) Cellular immunology 160, 24-32. PMID: 7842483

8. Yamazaki, T. et al. (1997) Journal of leukocyte biology 61, 73-9. PMID: 9000539

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CTSG polyclonal antibody at 1ug/mL on recombinant CTSG (Catalog No. HY284012).

References

Datasheet

Anti-CTSG Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CTSG Polyclonal Antibody (HY284014)

Overview

Images

References

Datasheet

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