Human PDHA1 Recombinant Protein (N-His) (HY552012)

Name: Human PDHA1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY552012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY552012
Description	Recombinant Human PDHA1 Protein, N-His (HY552012) expressed in E. coli, spanning Phe30-Ser390. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P08559
Protein length	Phe30-Ser390
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PHE1A, PDHE1-A type I, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHA1
Background	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) is a ~43 kDa protein. Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex. The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2. 1. Seifert, F. et al. (2007) Biochemistry 46, 6277-87. PMID: 17474719 2. Kato, M. et al. (2008) Structure (London, England : 1993) 16, 1849-59. PMID: 19081061 3. Korotchkina, LG. et al. (1995) The Journal of biological chemistry 270, 14297-304. PMID: 7782287
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PDHA1 Protein, N-His

References

HY552012-SDS-PAGE-1.jpg

Datasheet

Human PDHA1 Recombinant Protein (N-His).pdf

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Human PDHA1 Recombinant Protein (N-His) (HY552012)

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