Anti-PDHA1 Polyclonal Antibody (HY552014)

Name: Anti-PDHA1 Polyclonal Antibody
Brand: abinScience
SKU: HY552014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
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Overview

Catalog No.

HY552014

Description

Anti-PDHA1 Polyclonal Antibody (HY552014) is a rabbit polyclonal antibody detecting PDHA1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Macaca fascicularis, Pan troglodytes, Pongo abelii, Sminthopsis macroura, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDHA1 (Phe30-Ser390).

Target

PHE1A, PDHE1-A type I, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHA1

Purification

Purified by antigen affinity column.

Accession

P08559

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) is a ~43 kDa protein. Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex. The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2.

1. Seifert, F. et al. (2007) Biochemistry 46, 6277-87. PMID: 17474719

2. Kato, M. et al. (2008) Structure (London, England : 1993) 16, 1849-59. PMID: 19081061

3. Korotchkina, LG. et al. (1995) The Journal of biological chemistry 270, 14297-304. PMID: 7782287

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PDHA1 polyclonal antibody at 1ug/mL on recombinant PDHA1 (Catalog No. HY552012).

References

Datasheet

Anti-PDHA1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PDHA1 Polyclonal Antibody (HY552014)

Overview

Images

References

Datasheet

SDS

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