Human MAP1LC3C Recombinant Protein (N-His) (HP758012)

Name: Human MAP1LC3C Recombinant Protein (N-His)
Brand: abinScience
SKU: HP758012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP758012
Description	Recombinant Human MAP1LC3C Protein, N-His (HP758012) expressed in E. coli, spanning Arg11-Leu147. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BXW4
Protein length	Arg11-Leu147
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.07 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Microtubule-associated proteins 1A/1B light chain 3C, MAP1A/MAP1B LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1A/MAP1B light chain 3 C, Microtubule-associated protein 1 light chain 3 gamma, MAP1 light chain 3-like protein 3, MAP1LC3C, Autophagy-related protein LC3 C
Background	Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C) is a ~16 kDa protein. Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruits all ATG8 family members to infecting bacteria such as S.typhimurium. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. 1. von Muhlinen, N. et al. (2012) Molecular cell 48, 329-42. PMID: 23022382 2. Liu, X. et al. (2017) The Journal of cell biology 216, 1301-1320. PMID: 28404643
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human MAP1LC3C Protein, N-His

References

HP758012-SDS-PAGE-1.jpg

Datasheet

Human MAP1LC3C Recombinant Protein (N-His).pdf

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Formula

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Human MAP1LC3C Recombinant Protein (N-His) (HP758012)

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