Human SNX2 Recombinant Protein (N-His) (HT141012)

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Overview

Catalog No.	HT141012
Description	Recombinant Human SNX2 Protein, N-His (HT141012) expressed in E. coli, spanning Asp125-Ser277. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60749
Protein length	Asp125-Ser277
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Sorting nexin-2, Transformation-related gene 9 protein, TRG-9, SNX2, TRG9
Background	Sorting nexin-2 (SNX2) is a ~58 kDa protein. Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). 1. Carlton, JG. et al. (2005) Journal of cell science 118, 4527-39. PMID: 16179610 2. Rojas, R. et al. (2007) Molecular and cellular biology 27, 1112-24. PMID: 17101778 3. van Weering, JR. et al. (2012) The EMBO journal 31, 4466-80. PMID: 23085988 4. Lieu, ZZ. et al. (2010) European journal of cell biology 89, 379-93. PMID: 20138391 5. Prosser, DC. et al. (2010) Traffic (Copenhagen, Denmark) 11, 1347-62. PMID: 20604901
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human SNX2 Protein, N-His

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HT141012-SDS-PAGE-1.jpg

Datasheet

Human SNX2 Recombinant Protein (N-His).pdf

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Formula

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Human SNX2 Recombinant Protein (N-His) (HT141012)

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