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Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His (JN085022)

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Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His

Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His

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Datasheet

Overview

Catalog No.	JN085022
Description	Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His (JN085022) expressed in E. coli, spanning Leu1850-Gly2710. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P16154
Protein length	Leu1850-Gly2710
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Clostridioides difficile
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	100.01 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	glycosylating toxin TcdA, tcdA
Background	Toxin A is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis. 1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489 2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482 3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409 4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930 5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825 6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706 7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His

References

Datasheet

Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

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Volume

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Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant Clostridioides difficile Toxin A/tcdA Protein, C-His