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Recombinant Human NDUFS3 Protein, N-His (HC180012)

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Overview
Catalog No.HC180012
Description
Recombinant Human NDUFS3 Protein, N-His (HC180012) expressed in E. coli, spanning Asn51-Glu225. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionO75489
Protein lengthAsn51-Glu225
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 22.69 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCI-30kD, Complex I-30kD, EC:7.1.1.2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH-ubiquinone oxidoreductase 30 kDa subunit, NDUFS3
Background

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial is a ~30 kDa protein. Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.

1. Bénit, P. et al. (2004) Journal of medical genetics 41, 14-7. PMID: 14729820
2. Lou, X. et al. (2018) Journal of human genetics 63, 1269-1272. PMID: 30140060
3. Jaokar, TM. et al. (2013) Biochimie 95, 2392-403. PMID: 24028823
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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