Anti-Human NDUFS3 Polyclonal Antibody (HC180014)

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Datasheet

Overview

Catalog No.

HC180014

Description

Anti-Human NDUFS3 Polyclonal Antibody (HC180014) is a rabbit polyclonal antibody detecting NDUFS3. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NDUFS3 (Asn51-Glu225).

Target

CI-30kD, Complex I-30kD, EC:7.1.1.2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH-ubiquinone oxidoreductase 30 kDa subunit, NDUFS3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O75489

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (NDUFS3) is a ~30 kDa protein. Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.

1. Bénit, P. et al. (2004) Journal of medical genetics 41, 14-7. PMID: 14729820

2. Lou, X. et al. (2018) Journal of human genetics 63, 1269-1272. PMID: 30140060

3. Jaokar, TM. et al. (2013) Biochimie 95, 2392-403. PMID: 24028823

Note

For research use only

Images

References

Datasheet

Anti-Human NDUFS3 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NDUFS3 Polyclonal Antibody (HC180014)

Overview

Images

References

Datasheet

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