Human WIPI1 Recombinant Protein (N-His) (HD292012)

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Catalog No.	HD292012
Description	Recombinant Human WIPI1 Protein, N-His (HD292012) expressed in E. coli, spanning Leu17-Leu360. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5MNZ9
Protein length	Leu17-Leu360
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	40.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Atg18 protein homolog, WD repeat domain phosphoinositide-interacting protein 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI-1, WIPI1, WIPI49
Background	WD repeat domain phosphoinositide-interacting protein 1 is a ~48 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. 1. Proikas-Cezanne, T. et al. (2004) Oncogene 23, 9314-25. PMID: 15602573 2. Grotemeier, A. et al. (2010) Cellular signalling 22, 914-25. PMID: 20114074 3. Geeraert, C. et al. (2010) The Journal of biological chemistry 285, 24184-94. PMID: 20484055 4. Itakura, E. et al. (2010) Autophagy 6, 764-76. PMID: 20639694 5. Gaugel, A. et al. (2012) Journal of molecular signaling 7, 16. PMID: 23088497 6. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066 7. Maeda, S. et al. (2019) eLife 8. PMID: 31271352 8. Kojima, W. et al. (2021) Autophagy 17, 2011-2036. PMID: 33499712
Note	For research use only

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Human WIPI1 Recombinant Protein (N-His).pdf

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Human WIPI1 Recombinant Protein (N-His) (HD292012)

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