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Anti-BVDV N-terminal protease Polyclonal Antibody (VK778074)

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Overview
Catalog No.VK778074
Description
Anti-BVDV N-terminal protease Polyclonal Antibody (VK778074) is a rabbit polyclonal antibody detecting BVDV N-terminal protease in WB, IHC, ELISA.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityBovine viral diarrhea virus (BVDV) (Mucosal disease virus)
ApplicationsWB,IHC,ELISA
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant BVDV N-terminal protease (Met1-Cys168).
Target N-pro
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P19711
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesGenome polyprotein, N-terminal protease, N-pro, EC:3.4.22.-, Autoprotease p20, Capsid protein C, p14, E(rns) glycoprotein, gp44/48, EC:4.6.1.19, Envelope glycoprotein E1, gp33, Envelope glycoprotein E2, gp55, Viroporin p7, Non-structural protein 2-3, Cysteine protease NS2, EC:3.4.22.-, Non-structural protein 2, p54, Serine protease NS3, EC:3.4.21.113, EC:3.6.1.15, EC:3.6.4.13, Non-structural protein 3, p80, Non-structural protein 4A, NS4A, p10, Non-structural protein 4B, NS4B, p30, Non-structural protein 5A, NS5A, p58, RNA-directed RNA polymerase, EC:2.7.7.48, NS5B, p75
Background

Genome polyprotein (N-pro) is a ~449 kDa protein. Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.

1. Chen, Z. et al. (2007) Virology 366, 277-92. PMID: 17531282
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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