Anti-Influenza A virus NP/Nucleoprotein protein Nanobody (SAA1425) (VK528043)

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Datasheet

Overview

Catalog No.

VK528043

Description

Anti-Influenza A virus NP/Nucleoprotein protein Nanobody (SAA1425) [SAA1425] (VK528043) is a alpaca monoclonal antibody detecting Nucleoprotein in ELISA, FCM, IF.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus(strain A/WS/1933 H1N1)) (Q1K9H2), Influenza A virus (A/WSN/1933(H1N1)) (Q9DLK6)

Applications

ELISA, FCM, IF

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1425

Clonality

Monoclonal

Target

Nucleoprotein, Nucleocapsid protein, Protein N, NP

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P15682

RRID

Anti-Influenza A virus NP/Nucleoprotein protein Nanobody (SAA1425) (abinScience Cat# VK528043, RRID:AB_3726943)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

Note

For research use only.

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