Anti-Influenza A virus NP/Nucleoprotein protein Recombinant Antibody (VHH1) (VK528053)

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SDS

Overview

Catalog No.

VK528053

Description

Anti-Influenza A virus NP/Nucleoprotein protein Antibody (VHH1) [VHH1] (VK528053) is a mouse monoclonal antibody detecting Nucleoprotein in ELISA, FCM, IF.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus(strain A/WS/1933 H1N1)) (Q1K9H2), Influenza A virus (A/WSN/1933(H1N1)) (Q9DLK6)

Applications

ELISA, FCM, IF

Host species

Mouse

Isotype

VHH-mFc

Clone ID

VHH1

Clonality

Monoclonal

Target

NP, Nucleoprotein, Nucleocapsid protein, Protein N

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P15682

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

Note

For research use only.

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