Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014)

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Overview

Catalog No.

JN854014

Description

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014) is a rabbit polyclonal antibody detecting Arylesterase in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Pseudomonas fluorescens

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Pseudomonas fluorescens estF/Arylesterase (Met1-Arg272).

Target

Arylesterase, 3.1.1.2, Aryl-ester hydrolase, Carboxylic acid perhydrolase, PFE, Putative bromoperoxidase, 1.-.-.-, estF

Purification

Purified by antigen affinity column.

Accession

P22862

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Arylesterase (estF) is a ~30 kDa protein. Hydrolyzes phenolic esters, such as phenyl acetate, nitrophenyl acetate and naphtyl acetate. Can act on a wide range of esters, but reaction rate and enantioselectivity differ significantly depending on the substrate. Shows a preference for esters with small acyl groups. Also shows low perhydrolase activity, and catalyzes the reversible formation of peroxycarboxylic acids from carboxylic acids and hydrogen peroxide. In vitro, enzyme-generated peracetic acid oxidizes bromide ion to bromonium, which reacts with monochlorodimedone to form bromochlorodimedone.

1. Choi, KD. et al. (1990) Agricultural and biological chemistry 54, 2039-45. PMID: 1368608

2. Bernhardt, P. et al. (2005) Angewandte Chemie (International ed. in English) 44, 2742-2746. PMID: 15803517

3. Pelletier, I. et al. (1995) Microbiology (Reading, England) 141 ( Pt 2), 459-68. PMID: 7704276

4. Krebsfänger, N. et al. (1998) Journal of biotechnology 60, 105-11. PMID: 9571805

5. Cheeseman, JD. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 1237-43. PMID: 15213385

6. Yin, DL. et al. (2010) Biochemistry 49, 1931-42. PMID: 20112920

7. Yin, DT. et al. (2012) Chemistry (Weinheim an der Bergstrasse, Germany) 18, 8130-9. PMID: 22618813

Note

For research use only.

Images

References

Datasheet

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Pseudomonas fluorescens estF/Arylesterase Polyclonal Antibody (JN854014)

Overview

Images

References

Datasheet

SDS

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