ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Pseudomonas fluorescens
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Arylesterase (estF) is a ~30 kDa protein. Hydrolyzes phenolic esters, such as phenyl acetate, nitrophenyl acetate and naphtyl acetate. Can act on a wide range of esters, but reaction rate and enantioselectivity differ significantly depending on the substrate. Shows a preference for esters with small acyl groups. Also shows low perhydrolase activity, and catalyzes the reversible formation of peroxycarboxylic acids from carboxylic acids and hydrogen peroxide. In vitro, enzyme-generated peracetic acid oxidizes bromide ion to bromonium, which reacts with monochlorodimedone to form bromochlorodimedone.
1. Choi, KD. et al. (1990) Agricultural and biological chemistry 54, 2039-45. PMID: 1368608
2. Bernhardt, P. et al. (2005) Angewandte Chemie (International ed. in English) 44, 2742-2746. PMID: 15803517
3. Pelletier, I. et al. (1995) Microbiology (Reading, England) 141 ( Pt 2), 459-68. PMID: 7704276
4. Krebsfänger, N. et al. (1998) Journal of biotechnology 60, 105-11. PMID: 9571805
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