Clostridioides difficile Toxin B/tcdB Recombinant Protein (N-His-SUMO) (JN876022)

Name: Clostridioides difficile Toxin B/tcdB Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: JN876022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN876022
Description	Recombinant Clostridioides difficile Toxin B/tcdB Protein, N-His-SUMO (JN876022) expressed in E. coli, spanning Gly1834-Glu2053. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P18177
Protein length	Gly1834-Glu2053
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Clostridioides difficile
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	43.08 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Toxin B, Glucosyltransferase TcdB, tcdB, toxB, Clostridium difficile Toxin
Background	Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis. 1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489 2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149 3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482 4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933 5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463 6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936 7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356 9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706
Note	For research use only.