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HTNV/KHFV Glycoprotein N/Gn/G1 Recombinant Protein (C-His) (VK646061)

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Overview

Catalog No.	VK646061
Description	Recombinant HTNV/KHFV Glycoprotein N/Gn/G1, C-His (VK646061) expressed in Mammalian Cells. Purity: >90% as determined by SDS-PAGE.. Highlights ●High Purity — >90% as determined by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P08668
Protein length	Leu19-Glu371
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Envelopment polyprotein, GP, Gc, Glycoprotein C, Glycoprotein G1, Glycoprotein G2, Glycoprotein N, Glycoprotein precursor, Gn, M polyprotein
Background	Envelopment polyprotein is a ~126 kDa protein. Forms homotetramers with glycoprotein C at the surface of the virion. Attaches the virion to host cell receptors including integrin ITGAV/ITGB3. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. May also bind to host C1QBP for virus entry into the host cell. Mediates the assembly and budding of infectious virus particles through its interaction with the nucleocapsid protein and the viral genome. 1. Raymond, T. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1163-8. PMID: 15657120 2. Mou, DL. et al. (2006) Biochemical and biophysical research communications 339, 611-7. PMID: 16310165 3. Müller, A. et al. (2019) Virus research 267, 36-40. PMID: 31054291 4. Jin, M. et al. (2002) Virology 294, 60-9. PMID: 11886265 5. Choi, Y. et al. (2008) Virology 381, 178-83. PMID: 18834607 6. Wang, K. et al. (2019) Cell reports 27, 2075-2091.e5. PMID: 31091447
Note	For research use only

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Datasheet

HTNV/KHFV Glycoprotein N/Gn/G1 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Working Solution

C₂ (Working Conc.)

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HTNV/KHFV Glycoprotein N/Gn/G1 Recombinant Protein (C-His)