Anti-Human Transferrin R/TfR Reference Antibody (JST-TFR09, RUO) (HY036096)

Name: Anti-Human Transferrin R/TfR Reference Antibody (JST-TFR09, RUO)
Brand: abinScience
SKU: HY036096
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY036096

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Human

Isotype

IgG1

Clone ID

JST-TFR09

Biological activity

JST-TfR09 (10 ng/well-200 ng; 48 h) reduces viability of MT2, Su9T1, and KK1, and this cell death is inhibited by ferrostatin-1 [1]. JST-TfR09 (100 ng/mL; 48 h) downregulates TFR1, FTH1, and FTL, upregulates IRP2 and NCOA4, and induces lysosome-dependent ferritin degradation and autophagy in ED, KK1, and MT2 ATLL cell lines [1]. JST-TfR09 (100 ng/mL; 48 h) increases intracellular ferrous iron, ROS, and lipid peroxidation (MDA) levels, key hallmarks of ferroptosis, in Su9T1, MT2, ST1, SO4, KK1, and ED ATLL cell lines and MOLT4, JURKAT T-ALL cell lines [1]. JST-TfR09 induces in vitro toxicity to human erythroblasts differentiated from CD34 hematopoietic stem cells at concentrations greater than 156 ng/mL [2]. . Increased cell viability to almost 80% in MT2, Su9T1, and KK1 ATLL cell lines when co-treated with ferrostatin-1. Western Blot Analysis [1] Cell Line: ATLL cell lines (ED, KK1, MT2) Concentration: 100 ng/mL; 25 uM Aloxistatin () + 50 uM Pepstatin A () (pretreatment); 10 uM MG132 () (pretreatment) Incubation Time: 48 h (JST-TfR09); 24 h (pretreatment prior to JST-TfR09) Result: Decreased TFR1, FTH1, and FTL protein levels in ED and KK1 cells. Increased IRP2 and NCOA4 protein levels in ED and KK1 cells. Prevented JST-TfR09-induced ferritin degradation in cells pretreated with lysosomal inhibitors, while proteasomal inhibitor pretreatment did not. Increased LC3-II expression relative to LC3-I in cells, indicating autophagy activation, which was blocked by lysosomal inhibitor co-treatment.

Expression system

Mammalian cells

Clonality

Monoclonal

Target

Transferrin receptor protein 1, TfR1, T9, Trfr, p90, CD71, TFRC, TfR, sTfR, TR

Endotoxin level

Please contact the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02786

RRID

Anti-Human Transferrin R/TfR Reference Antibody (JST-TFR09, RUO) (abinScience Cat# HY036096, RRID:AB_3751763)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

Alternate Names

JST-TFR09, JSTTFR09, JSTTFR09

Background

Transferrin receptor protein 1 (CD71/TFRC) is a ~84 kDa protein. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

1. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738

2. Jabara, HH. et al. (2016) Nature genetics 48, 74-8. PMID: 26642240

References

1. Fauzi YR, et al. Anti-transferrin receptor antibody (JST-TFR09/PPMX-T003) induces ferroptosis in adult T-cell leukemia/lymphoma (ATLL) cells. Biochem Biophys Res Commun. 2025;756:151564. [HY036096]
2. Daniels-Wells TR, et al. Efficacy of Antibodies Targeting TfR1 in Xenograft Mouse Models of AIDS-Related Non-Hodgkin Lymphoma. Cancers (Basel). 2023;15(6):1816. Published 2023 Mar 17. [HY036096]

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD71/TFRC (JST-TFR09).
Flow CytoMetry

Flow-cytometry using APC anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD71 monoclonal antibody (Catalog: HY036096, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD71 monoclonal antibody (Catalog: HY036096, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD71 monoclonal antibody (Catalog: HY036096, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.