Anti-AIMP1 Polyclonal Antibody (HC554014)

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Overview

Catalog No.

HC554014

Description

Anti-AIMP1 Polyclonal Antibody (HC554014) is a rabbit polyclonal antibody detecting AIMP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Cricetulus griseus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AIMP1 (Lys148-Lys312).

Target

AIMP1, Endothelial monocyte-activating polypeptide II, EMAP-II, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP-2, SCYE1, EMAP2, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1

Purification

Purified by antigen affinity column.

Accession

Q12904

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1/EMAP-II) is a ~34 kDa protein. Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation.

1. Park, SG. et al. (1999) The Journal of biological chemistry 274, 16673-6. PMID: 10358004

2. Shalak, V. et al. (2001) The Journal of biological chemistry 276, 23769-76. PMID: 11306575

3. Han, JM. et al. (2006) Biochemical and biophysical research communications 342, 113-8. PMID: 16472771

4. Park, SG. et al. (2002) The Journal of biological chemistry 277, 45243-8. PMID: 12237313

5. Park, H. et al. (2002) Journal of leukocyte biology 71, 223-30. PMID: 11818442

6. Tandle, AT. et al. (2009) Experimental cell research 315, 1850-9. PMID: 19362550

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AIMP1 polyclonal antibody at 1ug/mL on recombinant AIMP1 (Catalog No. HC554012).

References

Datasheet

Anti-AIMP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-AIMP1 Polyclonal Antibody (HC554014)

Overview

Images

References

Datasheet

SDS

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