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Human AIMP1 Recombinant Protein (N-His) (HC554012)

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Human AIMP1 Recombinant Protein (N-His)

Human AIMP1 Recombinant Protein (N-His)

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Overview

Catalog No.	HC554012
Description	Recombinant Human AIMP1 Protein, N-His (HC554012) expressed in E. coli, spanning Lys148-Lys312. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q12904
Protein length	Lys148-Lys312
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.42 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AIMP1, Endothelial monocyte-activating polypeptide II, EMAP-II, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP-2, SCYE1, EMAP2, Multisynthase complex auxiliary component p43, Small inducible cytokine subfamily E member 1
Background	Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1/EMAP-II) is a ~34 kDa protein. Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. 1. Park, SG. et al. (1999) The Journal of biological chemistry 274, 16673-6. PMID: 10358004 2. Shalak, V. et al. (2001) The Journal of biological chemistry 276, 23769-76. PMID: 11306575 3. Han, JM. et al. (2006) Biochemical and biophysical research communications 342, 113-8. PMID: 16472771 4. Park, SG. et al. (2002) The Journal of biological chemistry 277, 45243-8. PMID: 12237313 5. Park, H. et al. (2002) Journal of leukocyte biology 71, 223-30. PMID: 11818442 6. Tandle, AT. et al. (2009) Experimental cell research 315, 1850-9. PMID: 19362550
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human AIMP1 Protein, N-His

References

HC554012-SDS-PAGE-1.jpg

Datasheet

Human AIMP1 Recombinant Protein (N-His).pdf

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Formula

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Human AIMP1 Recombinant Protein (N-His)