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Anti-Human DPEP3 Antibody (B43), FITC (HV426117)

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Anti-Human DPEP3 Antibody (B43), FITC

Anti-Human DPEP3 Antibody (B43), FITC

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Datasheet

Overview

Catalog No.	HV426117
Description	Anti-Human DPEP3 Antibody (B43), FITC [B43] (HV426117) is a mouse monoclonal antibody detecting DPEP3 in ELISA, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	B43
Conjugation	FITC
Clonality	Monoclonal
Target	DPEP3, Dipeptidase 3
Endotoxin level	Please contact with the lab for this information.
Purification	Affinity chromatography.
Accession	Q9H4B8
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Dipeptidase 3 (DPEP3) is a ~53 kDa protein. Lacks dipeptidase activity and is unable to hydrolyze cystinyl-bis-glycine, leukotriene D4 and the beta-lactam antibiotic imipenem. The absence of activity may be due to the inability of asparagine (instead of aspartate found in DPEP1/2) at position 359 to function as the acid/base catalyst and activate the nucleophilic water/hydroxide. A tyrosine (instead of histidine) at position 269 reduces affinity for the beta zinc and may cause substrate steric hindrance. 1. Hayashi, K. et al. (2020) Journal of structural biology 211, 107512. PMID: 32325220
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow Cytometry

Flow-cytometry using FITC anti-Human CD19 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-Human CD19 antibody monoclonal antibody (Catalog # HV426117 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human DPEP3 Antibody (B43), FITC.pdf

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Anti-Human DPEP3 Antibody (B43), FITC