Anti-Poliovirus Capsid protein VP3 Nanobody (7A) (VK557013)

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Overview
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Datasheet

Overview

Catalog No.

VK557013

Description

Anti-Poliovirus Capsid protein VP3 Nanobody (7A) [7A] (VK557013) is a alpaca monoclonal antibody detecting Capsid protein VP3 in ELISA, Neutralization.

Species reactivity

Poliovirus type 1 (strain Mahoney)

Applications

ELISA, Neutralization

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

Clonality

Monoclonal

Target

Genome polyprotein, Capsid protein VP3, Virion protein 3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P03300

RRID

Anti-Poliovirus Capsid protein VP3 Nanobody (7A) (abinScience Cat# VK557013, RRID:AB_3727139)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Genome polyprotein (Capsid protein VP3) is a ~246 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host epithelial cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

1. Hogle, JM. et al. (1985) Science (New York, N.Y.) 229, 1358-65. PMID: 2994218

2. Strauss, M. et al. (2013) Journal of virology 87, 3903-14. PMID: 23365424

3. Strauss, M. et al. (2015) Journal of virology 89, 4143-57. PMID: 25631086

4. Brandenburg, B. et al. (2007) PLoS biology 5, e183. PMID: 17622193

5. Coyne, CB. et al. (2007) The EMBO journal 26, 4016-28. PMID: 17717529

6. Bergelson, JM. (2008) Trends in microbiology 16, 44-7. PMID: 18191571

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Poliovirus Capsid protein VP3 Nanobody (7A)

References

Datasheet

Anti-Poliovirus Capsid protein VP3 Nanobody (7A).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Poliovirus Capsid protein VP3 Nanobody (7A) (VK557013)

Overview

Images

References

Datasheet

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