Anti-Human ASGR1/ASGPR1 Reference Antibody (AMG 529, RUO) (HY525016)

Name: Anti-Human ASGR1/ASGPR1 Reference Antibody (AMG 529, RUO)
Brand: abinScience
SKU: HY525016
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HY525016
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	AMG 529
Biological activity	AMG-529 induces a dose-dependent increase in alkaline phosphatase (ALP) levels, but no significant effect on blood lipid levels (including non-high-density lipoprotein cholesterol) is observed [1].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	HL-1, ASGP-R 1, Asialoglycoprotein receptor 1, CLEC4H1, ASGR1, C-type lectin domain family 4 member H1, ASGPR 1, Hepatic lectin H1
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P07306
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AMG-529, AMG529, AMG529
Background	Asialoglycoprotein receptor 1 (ASGR1) is a ~33 kDa protein. Transmembrane protein predominantly expressed on hepatocytes that plays a key role in endocytosis of plasma glycoproteins that lack terminal sialic acid residues. Specifically recognizes terminal galactose and N-acetylgalactosamine residues, facilitating the clearance of desialylated glycoproteins from circulation. Plays thereby a role in a variety of physiological processes, such as removal of desialylated platelets, elimination of activated lymphocytes and maintenance of serum glycoprotein homeostasis. Plays a role in the removal of desialylated platelets by activating a feedback loop regulating thrombopoietin (TPO) expression via the JAK2-STAT3 signaling pathway. Contributes also to recognition and elimination of activated lymphocytes by hepatocytes that can act as cytotoxic effectors. 1. Grozovsky, R. et al. (2015) Nature medicine 21, 47-54. PMID: 25485912 2. Guy, CS. et al. (2011) Hepatology (Baltimore, Md.) 54, 1043-50. PMID: 21656538
References	1. Janiszewski M, et al. A randomized, placebo-controlled, double-blind, ascending single-dose, phase 1 study to evaluate the safety, tolerability, pharmacokinetics, and pharmacodynamics of AMG 529, a novel anti-ASGR1 monoclonal antibody, in healthy subjects. Journal of the American College of Cardiology. 2019 Mar 12;73(9S1):1755-. [HY525016]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human ASGR1 (AMG 529).
Flow CytoMetry

Flow-cytometry using APC anti-human ASGR1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human ASGR1 monoclonal antibody (Catalog: HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human ASGR1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human ASGR1 monoclonal antibody (Catalog: HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human ASGR1 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ASGR1 monoclonal antibody (Catalog: HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human ASGR1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ASGR1 monoclonal antibody (Catalog HY525016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.