Anti-RELA Polyclonal Antibody (HC453014)

Name: Anti-RELA Polyclonal Antibody
Brand: abinScience
SKU: HC453014
Price: 96 USD
Availability: InStock

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Datasheet
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Overview

Catalog No.

HC453014

Description

Anti-RELA Polyclonal Antibody (HC453014) is a rabbit polyclonal antibody detecting RELA in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RELA (Met1-Gln220).

Target

Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65, NFKB3

Purification

Purified by antigen affinity column.

Accession

Q04206

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor p65 (RELA/NFkB p65) is a ~60 kDa protein. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

1. Bettelli, E. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 5138-43. PMID: 15790681

2. Yin, X. et al. (2021) Cell reports 34, 108628. PMID: 33440148

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RELA polyclonal antibody at 1ug/mL on recombinant RELA (Catalog No. HC453012).

References

Datasheet

Anti-RELA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-RELA Polyclonal Antibody (HC453014)

Overview

Images

References

Datasheet

SDS

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