Human BTK Recombinant Protein (N-His) (HC260012)

Name: Human BTK Recombinant Protein (N-His)
Brand: abinScience
SKU: HC260012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC260012
Description	Recombinant Human BTK Protein, N-His (HC260012) expressed in E. coli, spanning Arg28-Thr184. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q06187
Protein length	Arg28-Thr184
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.77 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BPK, Tyrosine-protein kinase BTK, AGMX1, Agammaglobulinemia tyrosine kinase, Bruton tyrosine kinase, BTK, ATK, B-cell progenitor kinase
Background	Tyrosine-protein kinase BTK is a ~76 kDa protein. Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. 1. Mohamed, AJ. et al. (2009) Immunological reviews 228, 58-73. PMID: 19290921 2. Rodriguez, R. et al. (2001) The Journal of biological chemistry 276, 47982-92. PMID: 11606584 3. Horwood, NJ. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 3635-41. PMID: 16517732 4. Doyle, SL. et al. (2007) The Journal of biological chemistry 282, 36953-60. PMID: 17932028
Note	For research use only.

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SDS-PAGE for Human BTK Recombinant Protein (N-His)

References

HC260012-SDS-PAGE-1.jpg

Datasheet

Human BTK Recombinant Protein (N-His).pdf

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Human BTK Recombinant Protein (N-His) (HC260012)

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