ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
15.79 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3, Mitogen-activated protein kinase kinase 1-interacting protein 1, Ragulator complex protein LAMTOR3, LAMTOR3, MAP2K1IP1, MAPKSP1, MEK-binding partner 1, Mitogen-activated protein kinase scaffold protein 1, Mp1
Background
Ragulator complex protein LAMTOR3 is a ~13 kDa protein. As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
1. Sancak, Y. et al. (2010) Cell 141, 290-303. PMID: 20381137
2. Bar-Peled, L. et al. (2012) Cell 150, 1196-208. PMID: 22980980
3. de Araujo, MEG. et al. (2017) Science (New York, N.Y.) 358, 377-381. PMID: 28935770
4. Su, MY. et al. (2017) Molecular cell 68, 835-846.e3. PMID: 29107538
5. Zhang, T. et al. (2017) Nature communications 8, 1394. PMID: 29123114
6. Yonehara, R. et al. (2017) Nature communications 8, 1625. PMID: 29158492
7. Shen, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 9545-9550. PMID: 30181260
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human LAMTOR3 Protein, N-His
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