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Anti-Thermus thermophilus SecY Recombinant Antibody (SAA1468) (JN047013)

Anti-Thermus thermophilus SecY Recombinant Antibody (SAA1468)
Anti-Thermus thermophilus SecY Recombinant Antibody (SAA1468)
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Overview
Catalog No.JN047013
Species reactivityThermus thermophilus
ApplicationsELISA
Host speciesMouse
IsotypeIgG2a
Clone IDSAA1468
Clonality Monoclonal
Target Protein translocase subunit SecY, Sec61α
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q5SHQ8
RRID Anti-Thermus thermophilus SecY Antibody (SAA1468) (abinScience Cat# JN047013, RRID:AB_3727105)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Protein translocase subunit SecY is a ~48 kDa protein. The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open.

Note For research use only.
Images
  • Anti-Thermus thermophilus SecY Recombinant Antibody (SAA1468)

    SDS-PAGE

    SDS-PAGE for Anti-Thermus thermophilus SecY Antibody (SAA1468)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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