Anti-Sahara scorpion AaH II/Neurotoxin II Nanobody (SAA2039) (ZA079023)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

ZA079023

Description

Anti-Sahara scorpion AaH II/Neurotoxin II Nanobody (SAA2039) [SAA2039] (ZA079023) is a alpaca monoclonal antibody detecting Neurotoxin II in ELISA, SPR.

Species reactivity

Androctonus australis (Sahara scorpion)

Applications

ELISA, SPR

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA2039

Clonality

Monoclonal

Target

Alpha-mammal toxin AaH2, AaHII, Neurotoxin 2, Neurotoxin II

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P01484

RRID

Anti-Sahara scorpion AaH II/Neurotoxin II Nanobody (SAA2039) (abinScience Cat# ZA079023, RRID:AB_3728995)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Alpha-mammal toxin AaH2 (AaH II/Neurotoxin II) is a ~9 kDa protein. Alpha toxin that binds voltage-independently at site-3 of sodium channels (Nav), inhibits the inactivation of the activated channels, and weakly inhibits activation, thereby blocking neuronal transmission. Inserts into voltage-sensing domain IV to stabilize a deactivated state, thereby preventing fast-inactivation. Principally slows the inactivation process of TTX-sensitive sodium channels. It is active on mammalian brain Nav1.2/SCN2A (EC(50)human=0.72 nM, EC(50)rat=2.6 nM), on rat skeletal muscle Nav1.4/SCN4A (EC(50)=2.2 nM), and on human neuronal Nav1.7/SCN9A (EC(50)=6.8-51.7 nM). In vivo, intraplantar injection into mice induces spontaneous pain responses.

1. Clairfeuille, T. et al. (2019) Science (New York, N.Y.) 363. PMID: 30733386

2. Abbas, N. et al. (2013) Pain 154, 1204-15. PMID: 23685008

3. Alami, M. et al. (2003) The Biochemical journal 375, 551-60. PMID: 12911331

Note

For research use only.

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