Human VDR Recombinant Protein (N-His) (HY304012)

Name: Human VDR Recombinant Protein (N-His)
Brand: abinScience
SKU: HY304012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY304012
Description	Recombinant Human VDR Protein, N-His (HY304012) expressed in E. coli, spanning Met272-Ser427. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P11473
Protein length	Met272-Ser427
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.02 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	VDR, Vitamin D3 receptor, 1, 25-dihydroxyvitamin D3 receptor, NR1I1, Nuclear receptor subfamily 1 group I member 1
Background	Vitamin D3 receptor (VDR) is a ~48 kDa protein. Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites. 1. Rochel, N. et al. (2000) Molecular cell 5, 173-9. PMID: 10678179 2. Eelen, G. et al. (2005) Molecular pharmacology 67, 1566-73. PMID: 15728261 3. Hourai, S. et al. (2006) Journal of medicinal chemistry 49, 5199-205. PMID: 16913708 4. Tamura, M. et al. (2017) Scientific reports 7, 5102. PMID: 28698609 5. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846 6. Makishima, M. et al. (2002) Science (New York, N.Y.) 296, 1313-6. PMID: 12016314 7. Hashimoto, N. et al. (2020) Kidney international 97, 1164-1180. PMID: 32354638
Note	For research use only.

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SDS-PAGE for Recombinant Human VDR Protein, N-His

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HY304012-SDS-PAGE-1.jpg

Datasheet

Human VDR Recombinant Protein (N-His).pdf

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Human VDR Recombinant Protein (N-His) (HY304012)

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