Human VPS4B Recombinant Protein (N-His) (HF794012)

Name: Human VPS4B Recombinant Protein (N-His)
Brand: abinScience
SKU: HF794012
Price: 211 USD
Availability: InStock

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Catalog No.	HF794012
Description	Recombinant Human VPS4B Protein, N-His (HF794012) expressed in E. coli, spanning Gly138-Gly444. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O75351
Protein length	Gly138-Gly444
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.25 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SKD1, VPS4B, Suppressor of K(+) transport growth defect 1, Cell migration-inducing gene 1 protein, Protein SKD1, Vacuolar protein sorting-associated protein 4B, VPS42
Background	Vacuolar protein sorting-associated protein 4B (VPS4B) is a ~49 kDa protein. Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan. 1. Lata, S. et al. (2008) Science (New York, N.Y.) 321, 1354-7. PMID: 18687924 2. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human VPS4B Protein, N-His

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HF794012-SDS-PAGE-1.jpg

Datasheet

Human VPS4B Recombinant Protein (N-His).pdf

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Human VPS4B Recombinant Protein (N-His) (HF794012)

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