Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320) (JN116013)

Name: Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320)
Brand: abinScience
SKU: JN116013
Price: 191 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN116013

Description

Anti-Escherichia coli ompA Nanobody (SAA2320) [SAA2320] (JN116013) is a human monoclonal antibody detecting OmpA in ELISA, FCM. Suitable for Escherichia coli.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Escherichia coli

Applications

ELISA, FCM

Host species

Human

Isotype

VHH-Fc

Clone ID

SAA2320

Clonality

Monoclonal

Target

Outer membrane protein A, OmpA, Outer membrane porin A, Outer membrane protein 3A, Outer membrane protein B, Outer membrane protein II*, Outer membrane protein d, ompA, con, tolG, tut

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0A910, P0A911

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Outer membrane protein A (ompA) is a ~37 kDa protein. With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm (Probable). Plays a role in resistance to environmental stress, and a role in outer membrane functionality and cell shape. Non-covalently binds peptidoglycan (Probable). Acts as a porin with low permeability that allows slow penetration of small solutes. A very abundant protein, there can be up to 210,000 OmpA molecules per cell.

1. Wang, Y. (2002) Biochemical and biophysical research communications 292, 396-401. PMID: 11906175

2. Sonntag, I. et al. (1978) Journal of bacteriology 136, 280-5. PMID: 361695

3. Ishida, H. et al. (2014) Biochimica et biophysica acta 1838, 3014-24. PMID: 25135663

4. Sugawara, E. et al. (1992) The Journal of biological chemistry 267, 2507-11. PMID: 1370823

5. Negoda, A. et al. (2010) Biochimica et biophysica acta 1798, 1480-4. PMID: 20004640

6. Negoda, A. et al. (2010) The FEBS journal 277, 4427-37. PMID: 21069910

7. Li, GW. et al. (2014) Cell 157, 624-35. PMID: 24766808

8. Sugawara, E. et al. (1994) The Journal of biological chemistry 269, 17981-7. PMID: 7517935

9. Arora, A. et al. (2000) The Journal of biological chemistry 275, 1594-600. PMID: 10636850

10. Zakharian, E. et al. (2005) Biochemistry 44, 6701-7. PMID: 15850404

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Escherichia coli ompA Nanobody (SAA2320)

References

Datasheet

Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Escherichia coli ompA Recombinant Nanobody (SAA2320) (JN116013)

Overview

Images

References

Datasheet

SDS

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