Anti-Human GBA Recombinant Nanobody (SAA2277) (HY292013)

Name: Anti-Human GBA Recombinant Nanobody (SAA2277)
Brand: abinScience
SKU: HY292013
Price: 191 USD
Availability: InStock

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100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY292013

Description

Anti-Human GBA Nanobody (SAA2277) [SAA2277] (HY292013) is a alpaca monoclonal antibody detecting Human GBA in ELISA, BLI. Suitable for Human.

Species reactivity

Human

Applications

ELISA, BLI

Host species

Alpaca

Isotype

VHH-His

Clone ID

SAA2277

Clonality

Monoclonal

Target

Lysosomal acid glucosylceramidase, Lysosomal acid GCase, EC:3.2.1.45, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-GC, Beta-glucosylceramidase 1, Cholesterol glucosyltransferase, SGTase, EC:2.4.1.-, Cholesteryl-beta-glucosidase, EC:3.2.1.-, D-glucosyl-N-acylsphingosine glucohydrolase, Glucosylceramidase beta 1, Imiglucerase, Lysosomal cholesterol glycosyltransferase, Lysosomal galactosylceramidase, EC:3.2.1.46, Lysosomal glycosylceramidase, GBA1

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P04062

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lysosomal acid glucosylceramidase (GBA) is a ~59 kDa protein. Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (such as N-acylsphing-4-enine) and glucose. Plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation. Catalyzes the glucosylation of cholesterol, through a transglucosylation reaction where glucose is transferred from GlcCer to cholesterol. GlcCer containing mono-unsaturated fatty acids (such as beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred as glucose donors for cholesterol glucosylation when compared with GlcCer containing same chain length of saturated fatty acids (such as beta-D-glucosyl-N-octadecanoyl-sphing-4-enine).

1. Ron, I. et al. (2005) Blood cells, molecules & diseases 35, 57-65. PMID: 15916907

2. Akiyama, H. et al. (2013) Biochemical and biophysical research communications 441, 838-43. PMID: 24211208

3. Akiyama, H. et al. (2020) The Journal of biological chemistry 295, 5257-5277. PMID: 32144204

4. Bannink, S. et al. (2024) Journal of lipid research 65, 100670. PMID: 39395789

5. Vaccaro, AM. et al. (1997) The Journal of biological chemistry 272, 16862-7. PMID: 9201993

6. Magalhaes, J. et al. (2016) Human molecular genetics 25, 3432-3445. PMID: 27378698

7. Kitatani, K. et al. (2009) The Journal of biological chemistry 284, 12972-8. PMID: 19279011

8. Marques, AR. et al. (2016) Journal of lipid research 57, 451-63. PMID: 26724485

9. Boer, DE. et al. (2021) Journal of lipid research 62, 100018. PMID: 33361282

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human GBA Nanobody (SAA2277)

References

Datasheet

Anti-Human GBA Recombinant Nanobody (SAA2277).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GBA Recombinant Nanobody (SAA2277) (HY292013)

Overview

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References

Datasheet

SDS

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