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Anti-Pembrolizumab Polyclonal Antibody (AS870014)

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Overview
Catalog No.AS870014
Description
Anti-Pembrolizumab Polyclonal Antibody (AS870014) is a rabbit polyclonal antibody detecting Pembrolizumab in ELISA.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityGeneral
ApplicationsELISA
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen Pembrolizumab
Target Rabbit polyclonal to Keytruda.
Specificity The product is specific for Pembrolizumab. This antibody serves as an excellent positive control for Pembrolizumab immunogenicity (ADA) assays.
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession CAS: 1374853-91-4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Programmed cell death protein 1 (Pembrolizumab) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

1. Fife, BT. et al. (2011) Annals of the New York Academy of Sciences 1217, 45-59. PMID: 21276005
2. Bardhan, K. et al. (2019) Scientific reports 9, 17252. PMID: 31754127
3. Patsoukis, N. et al. (2020) Communications biology 3, 128. PMID: 32184441
4. Xiao, X. et al. (2023) Nature communications 14, 2859. PMID: 37208329
5. Zak, KM. et al. (2015) Structure (London, England : 1993) 23, 2341-2348. PMID: 26602187
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Pembrolizumab Polyclonal Antibody

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