Anti-Pembrolizumab Polyclonal Antibody (AS870014)

Name: Anti-Pembrolizumab Polyclonal Antibody
Brand: abinScience
SKU: AS870014
Price: 168 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

AS870014

Description

Anti-Pembrolizumab Polyclonal Antibody (AS870014) is a rabbit polyclonal antibody detecting Pembrolizumab in ELISA.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

Applications

ELISA

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

Pembrolizumab

Target

Rabbit polyclonal to Keytruda.

Specificity

The product is specific for Pembrolizumab. This antibody serves as an excellent positive control for Pembrolizumab immunogenicity (ADA) assays.

Purification

Purified by antigen affinity column.

Accession

Q15116

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

The product can be stored for 2 - 3 weeks at 2 to 8°C or for up to 12 months at -20°C. Avoid repeated freezing and thawing cycles.

Background

Programmed cell death protein 1 (Pembrolizumab) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

1. Fife, BT. et al. (2011) Annals of the New York Academy of Sciences 1217, 45-59. PMID: 21276005

2. Bardhan, K. et al. (2019) Scientific reports 9, 17252. PMID: 31754127

3. Patsoukis, N. et al. (2020) Communications biology 3, 128. PMID: 32184441

4. Xiao, X. et al. (2023) Nature communications 14, 2859. PMID: 37208329

5. Zak, KM. et al. (2015) Structure (London, England : 1993) 23, 2341-2348. PMID: 26602187

Note

For research use only.

Images

References

Datasheet