Anti-PPIA Polyclonal Antibody (HX128014)

Name: Anti-PPIA Polyclonal Antibody
Brand: abinScience
SKU: HX128014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX128014

Description

Anti-PPIA Polyclonal Antibody (HX128014) is a rabbit polyclonal antibody detecting PPIA in ELISA, IHC, WB. Suitable for Human, Aotus trivirgatus, Bovine, Cricetulus griseus, and Cat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPIA (Val2-Glu165).

Target

PPIase A, Cyclosporin A-binding protein, Peptidyl-prolyl cis-trans isomerase A, Cyclophilin A, PPIA, CYPA, Rotamase A

Purification

Purified by antigen affinity column.

Accession

P62937

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peptidyl-prolyl cis-trans isomerase A (PPIA) is a ~18 kDa protein. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2.

1. Liu, J. et al. (1991) Biochemistry 30, 2306-10. PMID: 2001362

2. Davis, TL. et al. (2010) PLoS biology 8, e1000439. PMID: 20676357

3. Song, F. et al. (2011) The Journal of biological chemistry 286, 8197-8203. PMID: 21245143

4. Foster, TL. et al. (2011) Journal of virology 85, 7460-4. PMID: 21593166

5. Lauranzano, E. et al. (2015) Brain : a journal of neurology 138, 974-91. PMID: 25678563

6. Yurchenko, V. et al. (2002) The Journal of biological chemistry 277, 22959-65. PMID: 11943775

7. Jin, ZG. et al. (2004) Arteriosclerosis, thrombosis, and vascular biology 24, 1186-91. PMID: 15130913

8. Xie, Y. et al. (2019) Cellular signalling 61, 57-65. PMID: 31063815

9. Wei, Y. et al. (2013) Inflammation 36, 567-72. PMID: 23180369

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PPIA polyclonal antibody at 1ug/mL on recombinant PPIA (Catalog No. HX128012).

References

Datasheet

Anti-PPIA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-PPIA Polyclonal Antibody (HX128014)

Overview

Images

References

Datasheet

SDS

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