Anti-PRKAA1/AMPK alpha 1 Polyclonal Antibody (HC365014)

Name: Anti-PRKAA1/AMPK alpha 1 Polyclonal Antibody
Brand: abinScience
SKU: HC365014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC365014

Description

Anti-PRKAA1/AMPK alpha 1 Polyclonal Antibody (HC365014) is a rabbit polyclonal antibody detecting AMPK subunit alpha-1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PRKAA1/AMPK alpha 1 (Met10-Gln559).

Target

AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, Tau-protein kinase PRKAA1, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK1, PRKAA1, HMGCR kinase

Purification

Purified by antigen affinity column.

Accession

Q13131

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1/AMPK alpha 1) is a ~64 kDa protein. Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2).

1. Towler, MC. et al. (2007) Circulation research 100, 328-41. PMID: 17307971

2. Hardie, DG. (2007) Nature reviews. Molecular cell biology 8, 774-85. PMID: 17712357

3. Bullen, JW. et al. (2014) The Journal of biological chemistry 289, 10592-10606. PMID: 24563466

4. Yang, JS. et al. (2019) Nature communications 10, 4068. PMID: 31492851

5. Wu, N. et al. (2023) Virology journal 20, 231. PMID: 37821951

6. Yang, JS. et al. (2025) Cell 188, 2569-2585.e20. PMID: 40233740

7. Liu, R. et al. (2021) Molecular cell 81, 2722-2735.e9. PMID: 34077757

8. Yang, W. et al. (2001) The Journal of biological chemistry 276, 38341-4. PMID: 11518699

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PRKAA1/AMPK alpha 1 polyclonal antibody at 1ug/mL on recombinant PRKAA1/AMPK alpha 1 (Catalog No. HC365012).

References

Datasheet

Anti-PRKAA1/AMPK alpha 1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PRKAA1/AMPK alpha 1 Polyclonal Antibody (HC365014)

Overview

Images

References

Datasheet

SDS

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