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Anti-Yersinia pestis Plasminogen activator/Pla Polyclonal Antibody (JN173024)

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Overview
Catalog No.JN173024
Description
Anti-Yersinia pestis Plasminogen activator/Pla Polyclonal Antibody (JN173024) is a rabbit polyclonal antibody detecting Plasminogen activator. Suitable for Yersinia pestis.
Species reactivityYersinia pestis
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Yersinia pestis Plasminogen activator/Pla (Ala21-Phe312).
Target Coagulase/fibrinolysin, EC:3.4.23.48, Pla, Plasminogen activator, pla
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P17811
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Plasminogen activator is a ~34 kDa protein. In the mammalian host activates (cleaves) plasminogen to generate the serine protease plasmin. Plasmin degrades fibrin clots (fibrinolysis) and facilitates bacterial cell migration, enabling rapid dissemination of bacteria from the initial site of infection (Probable). Cleaves host plasminogen to generate plasmin and probably also has autocatalytic activity. Fibrinolytic activity prevails at 37 degrees Celsius whereas coagulase expression predominates at lower temperatures (28 degrees Celsius). Cleaves plasminogen; plasminogen cleavage is much higher than coagulase activity.

1. Eren, E. et al. (2010) Structure (London, England : 1993) 18, 809-18. PMID: 20637417
2. Eren, E. et al. (2012) The Journal of biological chemistry 287, 23971-6. PMID: 22645135
3. McDonough, KA. et al. (1989) Molecular microbiology 3, 767-75. PMID: 2526282
4. Sodeinde, OA. et al. (1989) Infection and immunity 57, 1517-23. PMID: 2651310
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Yersinia pestis Plasminogen activator/Pla Polyclonal Antibody

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