GMP Grade Human INHBA (Tag Free) (HW366022)

Name: GMP Grade Human INHBA (Tag Free)
Brand: abinScience
SKU: HW366022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW366022
Description	Recombinant Human CTCF Protein, N-His (HW366022) expressed in E. coli, spanning Thr265-Arg572. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P49711, Q61164, Q9R1D1
Protein length	Thr265-Arg572
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.93 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	11-zinc finger protein, CCCTC-binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF
Background	Transcriptional repressor CTCF is a ~82 kDa protein. Chromatin binding factor that binds to DNA sequence specific sites and regulates the 3D structure of chromatin. Binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures, such as the nuclear lamina. Defines the boundaries between active and heterochromatic DNA via binding to chromatin insulators, thereby preventing interaction between promoter and nearby enhancers and silencers. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. 1. Ishihara, K. et al. (2006) Molecular cell 23, 733-42. PMID: 16949368 2. Majumder, P. et al. (2008) The Journal of experimental medicine 205, 785-98. PMID: 18347100 3. Fu, Y. et al. (2008) PLoS genetics 4, e1000138. PMID: 18654629 4. Mishiro, T. et al. (2009) The EMBO journal 28, 1234-45. PMID: 19322193
Note	For research use only

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Datasheet

GMP Grade Human INHBA (Tag Free).pdf

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GMP Grade Human INHBA (Tag Free) (HW366022)

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