Human RECQ2/BLM Recombinant Protein (N-His) (HX157012)

Name: Human RECQ2/BLM Recombinant Protein (N-His)
Brand: abinScience
SKU: HX157012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX157012
Description	Recombinant Human RECQ2/BLM Protein, N-His (HX157012) expressed in E. coli, spanning Ser636-Cys1067. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P54132
Protein length	Ser636-Cys1067
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	51.92 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	BLM, Bloom syndrome protein, DNA 3'-5' helicase BLM, DNA helicase, RecQ-like type 2, EC:5.6.2.4, RECQ2, RECQL3, RecQ protein-like 3, RecQ-like DNA helicase BLM, RecQ2
Background	RecQ-like DNA helicase BLM is a ~159 kDa protein. ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction. Participates in DNA replication and repair. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA. 1. Swan, MK. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1465-75. PMID: 24816114 2. Newman, JA. et al. (2015) Nucleic acids research 43, 5221-35. PMID: 25901030 3. Karow, JK. et al. (1997) The Journal of biological chemistry 272, 30611-4. PMID: 9388193 4. Sun, H. et al. (1998) The Journal of biological chemistry 273, 27587-92. PMID: 9765292 5. Langland, G. et al. (2002) Cancer research 62, 2766-70. PMID: 12019152 6. Nimonkar, AV. et al. (2011) Genes & development 25, 350-62. PMID: 21325134 7. Wan, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10646-51. PMID: 23509288 8. Wu, C. et al. (2021) Nucleic acids research 49, 11224-11240. PMID: 34606619
Note	For research use only

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Datasheet

Human RECQ2/BLM Recombinant Protein (N-His).pdf

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Human RECQ2/BLM Recombinant Protein (N-His) (HX157012)

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