Human UVRAG Recombinant Protein (N-His) (HA311012)

Name: Human UVRAG Recombinant Protein (N-His)
Brand: abinScience
SKU: HA311012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA311012
Description	Recombinant Human UVRAG Protein, N-His (HA311012) expressed in E. coli, spanning Arg41-Ala164. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9P2Y5
Protein length	Arg41-Ala164
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.85 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	UV radiation resistance-associated gene protein, p63, UVRAG
Background	UV radiation resistance-associated gene protein (UVRAG) is a ~78 kDa protein. Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P). During autophagy acts as a regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2. 1. Liang, C. et al. (2006) Nature cell biology 8, 688-99. PMID: 16799551 2. Liang, C. et al. (2008) Nature cell biology 10, 776-87. PMID: 18552835 3. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123 4. He, S. et al. (2013) Nature cell biology 15, 1206-1219. PMID: 24056303 5. Cheng, X. et al. (2017) Molecular cell 65, 1029-1043.e5. PMID: 28306502
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human UVRAG Protein, N-His

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HA311012-SDS-PAGE-1.jpg

Datasheet

Human UVRAG Recombinant Protein (N-His).pdf

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Human UVRAG Recombinant Protein (N-His) (HA311012)

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