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Anti-Human CD45/PTPRC Antibody (BC8), PE (HY195127)

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Anti-Human CD45/PTPRC Antibody (BC8), PE

Anti-Human CD45/PTPRC Antibody (BC8), PE

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개요
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개요

카탈로그 번호	HY195127
설명	Anti-Human CD45/PTPRC Antibody (BC8), PE [BC8] (HY195127) is a mouse monoclonal antibody detecting PTPRC in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
종 반응성	Human
응용 분야	FCM
숙주 종	Mouse
이소타입	IgG1, kappa
클론 ID	BC8
접합	PE
클론성	Monoclonal
타겟	T200, Leukocyte common antigen, CD45, L-CA, Receptor-type tyrosine-protein phosphatase C, PTPRC
내독소 수준	Please contact with the lab for this information.
등록번호	P08575
형태	Liquid
저장 완충액	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Store at 4°C for 12 months. Protect from light. Do not freeze.
배경	Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~147 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. 1. Dunlock, VE. et al. (2022) Cell reports 39, 111006. PMID: 35767951
참고사항	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using PE anti-human CD45 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD45 monoclonal antibody (Catalog: HY195127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌

데이터시트

Anti-Human CD45/PTPRC Antibody (BC8), PE.pdf

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Anti-Human CD45/PTPRC Antibody (BC8), PE