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ELISA Kits

abinScience provides research-use ELISA kits spanning sandwich, competitive, and sVNT formats. Each lot includes LoD/LoQ, linearity, spike-recovery, and precision data. Validated for CSF, serum, plasma. 96-well breakable strips. RUO

Why Researchers Choose abinScience

Full Formats Sandwich, competitive, sVNT (ACE2–RBD)
Lot-Specific QC LoD/LoQ, linearity, CV, recovery
Matrix Protocols CSF, serum, plasma; MRD guidance
Ready-to-Use Calibrators, controls, reagents
Flexible Plates 96-well breakable strips

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Selection & Assay Guide

  1. Assay Format: Use sandwich for proteins; competitive for small molecules; sVNT for neutralizing antibody assessment (reports Inhibition%/IC50).
  2. Matrix: Follow MRD in CoA; validate spike-recovery (80–120%) if needed.
  3. Curve Fitting: Default 4PL/5PL; include full standard range and run controls on every plate.
  4. Performance Metrics: Check LoD/LoQ, linearity, recovery, and CV (typically ≤10–15%).
  5. Run Strategy: Use breakable 96-well strips; avoid freeze-thaw cycles; prepare working solutions per protocol.

Applications

Biomarker quantification in CSF/serum/plasma
Neutralizing antibody assessment via ACE2–RBD blocking (sVNT)
Method development, tech transfer and laboratory training

FAQs

What is the difference between sandwich and competitive ELISA?

Sandwich requires two non-overlapping epitopes and offers high sensitivity; competitive fits small molecules or single-epitope contexts and yields inverse signals.

How do LoD and LoQ differ?

LoD is the lowest detectable concentration; LoQ is the lowest quantifiable concentration meeting accuracy/precision criteria. Refer to the lot-specific CoA.

How is Inhibition% calculated for sVNT?

Inhibition% = [1 − (ODsample/ODnegative control)] × 100. Serial dilutions allow IC50/ID50 calculation.

Should I use 4PL or 5PL curve fitting?

Use 4PL by default; apply 5PL for asymmetric curves or tailing and verify residuals.

RUO disclaimer

All products are for Research Use Only (RUO) and not for diagnostic or therapeutic procedures.

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