Please ensure Javascript is enabled for purposes of website accessibility
Contact us Contact Us
EN
Home > Products > Antibodies > Isotype Controls

Isotype Controls

abinScience provides matched isotype control antibodies for flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC) and Western blot (WB). Each control is aligned to the host species, isotype/subclass, light chain (κ/λ) and conjugate of your primary antibody, with cross-adsorbed and F(ab')2 options to minimize non-specific binding. Lot-specific QC includes recommended dilutions, storage and application notes. RUO

Why Researchers Choose abinScience

Perfectly Matched Host, isotype, subclass, light chain, conjugate
Cross-Adsorbed Reduce off-target binding in multi-species
F(ab')2 Fragments Avoid FcR binding in macrophage-rich tissues
Bright Conjugates AF488/594/647, FITC, PE, APC, BV421, HRP/AP
Lot-Specific QC Dilution, fixation, example images

Shop by Category

Selection & Best Practices

  1. Match Precisely: Same host species, isotype/subclass (e.g., mouse IgG1 κ), and the same conjugate as your primary; use the same concentration or mass per test.
  2. Fragment Choice: Choose F(ab')2 when Fc receptors cause background (macrophage-rich tissues, spleen, tumors).
  3. Cross-Adsorption: Select cross-adsorbed (or highly cross-adsorbed) for multi-species samples to reduce off-target binding.
  4. Flow Gating: Use FMO controls to set positive gates; use isotypes to estimate non-specific binding—not to define thresholds.
  5. Fix/Perm Compatibility: Verify tandem dye and fixation compatibility (paraformaldehyde, methanol, saponin) on the datasheet.

Applications

Flow cytometry background estimation and panel QC
IHC/IF specificity checks in FcR-rich or sticky matrices
WB chemiluminescent controls (HRP/AP) for substrate checks

Documents & QC

Datasheet with host/isotype/subclass, light chain and conjugate
Cross-adsorption matrix; recommended dilutions; example images
Lot-specific CoA; storage, light protection and stability guidance

FAQs

Should I always use an isotype control?

Use FMO controls for gating in multicolor flow; isotypes help estimate non-specific binding from Fc/charge/hydrophobic interactions, especially in sticky tissues or when primaries are low-specificity.

How do I choose between whole IgG and F(ab')2?

Whole IgG maximizes signal; F(ab')2 lacks Fc to avoid FcR-mediated binding and often lowers background in IF/IHC and flow.

What “cross-adsorbed” means?

Antiserum is adsorbed against non-target species to reduce cross-reactivity; “highly cross-adsorbed” further minimizes off-target binding in multi-species samples.

RUO disclaimer

All antibodies are for Research Use Only and not for diagnostic procedures.

Options+
Options
Confirm
  • Product types
    0 selected
    Antibodies (385)
  • Target
    0 selected
    IgG1 (N297Q) (1)
    IgG2C (5)
    IgG2-lambda (6)
    IgG1 (K214R/L234A/L235A) (1)
    IgG4 (S228P/L235E/R409K) (1)
    IgG1 (K97R) (1)
    VHH-hFc (IgG4) (1)
    IgG1 (D265A) (2)
    IgG4 (S228P/R409K) (1)
    IgD-kappa (1)
    IgE (10)
    IgG1 (L234A/L235A/F405L) (1)
    VHH-mFc (IgG1) (1)
    IgG3 (11)
    IgG1 (E356D/M358L/L234A/L235A) (1)
    IgG4 (S228P/D265A/L309V/R409K) (1)
    IgG2b (D265A)-kappa (1)
    IgG1 (K214R/K447A) (1)
    IgG1 (M428L/N434S) (1)
    IgG1 (LALA) (1)
    IgG1-lambda (10)
    IgG1 CH1-IgG2 Fc Fragment (1)
    IgG4 (S228P) (8)
    IgG1 (E356D/M358L/L234A/L235A/F405L) (1)
    IgG4 (S228P/F234A/L235A/R409K) (1)
    IgG1 (N297S) (1)
    IgG1 (K214R/L234F/L235E/P331S) (1)
    scFv-hFc (IgG1) (1)
    IgG1 (LALA P329G) (1)
    IgG1 (S239D/A330L/I332E) (3)
    IgG1 (D265A/N297A) (1)
    IgG1 (L234A/L235A/G237A) (1)
    VHH-mFc (IgG2a) (1)
    IgG4 (S228P & L235E) (1)
    IgA2-lambda (1)
    IgG4-kappa (2)
    IgA2 (1)
    IgG1 (L234A/L235A) (2)
    IgG1-G2-kappa (1)
    IgG (46)
    IgG1 (LALAPG) (1)
    IgG2-kappa (5)
    IgG1 (E356D/M358L) (1)
    IgM-kappa (26)
    IgG1 (F405L) (1)
    IgG4 (S228P/L235E/D265A/R409K) (1)
    IgA (17)
    VHH (10)
    IgG1 (P329G/E356D/M358L) (1)
    IgG1 (K214R/N297A) (1)
    scFv-mFc (IgG2a) (1)
    IgG1 (D270N) (1)
    IgG4 (S228P/F234A/L235A) (2)
    IgG1 (D265A)-kappa (1)
    IgG1 (L234F/L235E) (1)
    IgG2a (28)
    IgG1 (48)
    VHH-mFc (5)
    IgG1 (L234F/L235E/P331S) (1)
    IgG1 (S239D & I332E) (1)
    IgG1 (Fab-His) (1)
    IgG1 (N297A/E356D/M358L) (1)
    IgG2a (L234A/L235A) (1)
    IgG2a (LALA) (1)
    IgG3-lambda (10)
    IgG1 (K214R/E356D/M358L) (1)
    IgG4 (S228P/L235E/P329G) (1)
    IgG1-Avi-Biotin (1)
    VHH-hFc (IgG1) (5)
    IgG1 (P331S/E430G) (1)
    BiTE (Bispecific) (1)
    IgG1 (K409R/E356D/M358L) (1)
    scFv (1)
    IgG1 fut (1)
    lgM-Lambda (5)
    IgG1 (E233A/L235A) (1)
    IgG1 (K214R) (1)
    IgG2b (28)
    IgG4-lambda (10)
    IgG1 (N297A) (3)
    IgG (Bispecific) (3)
    IgY (5)
    IgG2 (10)
    IgG2a (D265A)-kappa (1)
  • Applications
    0 selected
    ELISA (1)
    Isotype Control (377)
    IHC (1)
    Control (4)
    FCM (360)
    IF (5)
    In vivo Control (123)
  • Reactivity
    0 selected
    Dog (10)
    Cat (15)
  • Host species
    0 selected
    Llama (5)
    Human (116)
    Rat (57)
    Rabbit (11)
    Canine (18)
    Alpaca (10)
    Monkey (5)
    Mouse (106)
    Dog (20)
    Goat (5)
    Chicken (5)
    Guinea pig (5)
    Feline (20)
  • Clonality
    0 selected
    Polyclonal (45)
    Monoclonal (338)
    Human (1)
  • Conjugate
    0 selected
    Unconjugated (171)
    FITC (52)
    APC (51)
    PerCP (51)
    PE (51)

Quick access

Contact Us

  • +86-027-65523339

  • info@abinscience.com

  • Building C, No. 666, Shen Dun Si Lu, Wuhan, 430206, China

Copyright © 2025 abinScience. All Rights Reserved. All Products are for Research Use Only