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Isotype Controls

abinScience provides matched isotype control antibodies for flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC) and Western blot (WB). Each control is aligned to the host species, isotype/subclass, light chain (κ/λ) and conjugate of your primary antibody, with cross-adsorbed and F(ab')2 options to minimize non-specific binding. Lot-specific QC includes recommended dilutions, storage and application notes. RUO

Why Researchers Choose abinScience

Perfectly Matched Host, isotype, subclass, light chain, conjugate
Cross-Adsorbed Reduce off-target binding in multi-species
F(ab')2 Fragments Avoid FcR binding in macrophage-rich tissues
Bright Conjugates AF488/594/647, FITC, PE, APC, BV421, HRP/AP
Lot-Specific QC Dilution, fixation, example images

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Selection & Best Practices

  1. Match Precisely: Same host species, isotype/subclass (e.g., mouse IgG1 κ), and the same conjugate as your primary; use the same concentration or mass per test.
  2. Fragment Choice: Choose F(ab')2 when Fc receptors cause background (macrophage-rich tissues, spleen, tumors).
  3. Cross-Adsorption: Select cross-adsorbed (or highly cross-adsorbed) for multi-species samples to reduce off-target binding.
  4. Flow Gating: Use FMO controls to set positive gates; use isotypes to estimate non-specific binding—not to define thresholds.
  5. Fix/Perm Compatibility: Verify tandem dye and fixation compatibility (paraformaldehyde, methanol, saponin) on the datasheet.

Applications

Flow cytometry background estimation and panel QC
IHC/IF specificity checks in FcR-rich or sticky matrices
WB chemiluminescent controls (HRP/AP) for substrate checks

Documents & QC

Datasheet with host/isotype/subclass, light chain and conjugate
Cross-adsorption matrix; recommended dilutions; example images
Lot-specific CoA; storage, light protection and stability guidance

FAQs

Should I always use an isotype control?

Use FMO controls for gating in multicolor flow; isotypes help estimate non-specific binding from Fc/charge/hydrophobic interactions, especially in sticky tissues or when primaries are low-specificity.

How do I choose between whole IgG and F(ab')2?

Whole IgG maximizes signal; F(ab')2 lacks Fc to avoid FcR-mediated binding and often lowers background in IF/IHC and flow.

What “cross-adsorbed” means?

Antiserum is adsorbed against non-target species to reduce cross-reactivity; “highly cross-adsorbed” further minimizes off-target binding in multi-species samples.

RUO disclaimer

All antibodies are for Research Use Only and not for diagnostic procedures.

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