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Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014)

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Overview
Catalog No.HX129014
Description
Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody (HX129014) is a rabbit polyclonal antibody detecting POU5F1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Macaca mulatta, and Pan troglodytes.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human POU5F1/OCT3/OCT4 (Ser136-Ser289).
Target Octamer-binding protein 4, Oct-4, OCT3, OCT4, OTF3, OTF-3, Octamer-binding transcription factor 3, POU5F1, Oct-3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3
Purification Purified by antigen affinity column.
Accession Q01860
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

POU domain, class 5, transcription factor 1 (POU5F1/OCT3) is a ~38 kDa protein. Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Note For research use only.
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Formula
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Anti-POU5F1/OCT3/OCT4 Polyclonal Antibody

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