Anti-PTK2 Polyclonal Antibody (HC379014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HC379014

Description

Anti-PTK2 Polyclonal Antibody (HC379014) is a rabbit polyclonal antibody detecting PTK2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Chicken, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Chicken, Xenopus laevis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PTK2 (Gly798-Ala1041).

Target

p125FAK, pp125FAK, PPP1R71, Protein-tyrosine kinase 2, FADK 1, FAK1, Focal adhesion kinase 1, FAK, PTK2, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71

Purification

Purified by antigen affinity column.

Accession

Q05397

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Focal adhesion kinase 1 (PTK2) is a ~119 kDa protein. Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts.

1. Tachibana, K. et al. (1997) The Journal of biological chemistry 272, 29083-90. PMID: 9360983

Note

For research use only.

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Western blot

Western blot analysis was performed using anti-PTK2 polyclonal antibody at 1ug/mL on recombinant PTK2 (Catalog No. HC379012).

References

Datasheet

Anti-PTK2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PTK2 Polyclonal Antibody (HC379014)

Overview

Images

References

Datasheet

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