Anti-PTPN11 Polyclonal Antibody (HC400014)

Name: Anti-PTPN11 Polyclonal Antibody
Brand: abinScience
SKU: HC400014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC400014

Description

Anti-PTPN11 Polyclonal Antibody (HC400014) is a rabbit polyclonal antibody detecting PTPN11 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PTPN11 (Met1-Arg593).

Target

Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-2C, Shp2, SHP-2, SH-PTP3, Protein-tyrosine phosphatase 2C, PTP2C, PTP-1D, PTPN11, SHPTP2, SH-PTP2

Purification

Purified by antigen affinity column.

Accession

Q06124

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tyrosine-protein phosphatase non-receptor type 11 (PTPN11) is a ~68 kDa protein. Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73.

1. Miao, H. et al. (2000) Nature cell biology 2, 62-9. PMID: 10655584

2. Marshall, AS. et al. (2004) The Journal of biological chemistry 279, 14792-802. PMID: 14739280

3. Lee, HH. et al. (2008) The Journal of cell biology 181, 999-1012. PMID: 18559669

4. Jakob, S. et al. (2008) The Journal of biological chemistry 283, 33155-61. PMID: 18829466

5. Noda, S. et al. (2016) Biochemical and biophysical research communications 469, 1133-9. PMID: 26742426

6. Pannone, L. et al. (2017) Human mutation 38, 451-459. PMID: 28074573

7. Patsoukis, N. et al. (2020) Communications biology 3, 128. PMID: 32184441

Note

For research use only.

Images

References

Datasheet

Anti-PTPN11 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PTPN11 Polyclonal Antibody (HC400014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us