Anti-CD69 Polyclonal Antibody (HC273014)

Name: Anti-CD69 Polyclonal Antibody
Brand: abinScience
SKU: HC273014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC273014

Description

Anti-CD69 Polyclonal Antibody (HC273014) is a rabbit polyclonal antibody detecting CD69 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD69 (Ser62-Lys199).

Target

AIM, CD69, C-type lectin domain family 2 member C, Early T-cell activation antigen p60, BL-AC/P26, GP32/28, MLR-3, Early activation antigen CD69, Leukocyte surface antigen Leu-23, CLEC2C, EA1, Activation inducer molecule

Purification

Purified by antigen affinity column.

Accession

Q07108

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells.

1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896

2. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369

3. Jiménez-Fernández, M. et al. (2022) Cellular and molecular life sciences : CMLS 79, 468. PMID: 35930205

4. Jiang, Y. et al. (2021) Clinical and translational medicine 11, e563. PMID: 34841721

5. Chen, H. et al. (2023) eLife 12. PMID: 37039481

Note

For research use only.

Images

References

Datasheet

Anti-CD69 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD69 Polyclonal Antibody (HC273014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us