Anti-IL24 Polyclonal Antibody (HC472014)

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100ug

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Datasheet

Overview

Catalog No.

HC472014

Description

Anti-IL24 Polyclonal Antibody (HC472014) is a rabbit polyclonal antibody detecting IL24 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IL24 (Gln52-Leu206).

Target

MDA7, MDA-7, ST16, IL-24, Melanoma differentiation-associated gene 7 protein, Suppression of tumorigenicity 16 protein, IL24, Interleukin-24

Purification

Purified by antigen affinity column.

Accession

Q13007

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-24 (IL24) is a ~23 kDa protein. Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis. Possesses antiviral functions and induces the type I interferon response during influenza infection. Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1. In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1. Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway-mediated stress signal.

1. Jin, SH. et al. (2014) Toxicology and applied pharmacology 280, 199-206. PMID: 25168428

2. Seong, RK. et al. (2016) Journal of microbiology (Seoul, Korea) 54, 695-700. PMID: 27687232

3. Wang, M. et al. (2002) The Journal of biological chemistry 277, 7341-7. PMID: 11706020

4. Lubkowski, J. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 201, 2082-2093. PMID: 30111632

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IL24 polyclonal antibody at 1ug/mL on recombinant IL24 (Catalog No. HC472012).

References

Datasheet

Anti-IL24 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-IL24 Polyclonal Antibody (HC472014)

Overview

Images

References

Datasheet

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