Anti-SQSTM1/p62 Polyclonal Antibody (HC648014)

Name: Anti-SQSTM1/p62 Polyclonal Antibody
Brand: abinScience
SKU: HC648014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HC648014

Description

Anti-SQSTM1/p62 Polyclonal Antibody (HC648014) is a rabbit polyclonal antibody detecting SQSTM1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SQSTM1 (Met1-Val122).

Target

OSIL, EBIAP, Sequestosome-1, Ubiquitin-binding protein p62, ORCA, EBI3-associated protein of 60 kDa, p60, SQSTM1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Purification

Purified by antigen affinity column.

Accession

Q13501

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Sequestosome-1 (SQSTM1/p62) is a ~47 kDa protein. Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes. Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps. SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies. SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins. SQSTM1 is itself degraded along with its ubiquitinated cargos.

1. Seibenhener, ML. et al. (2004) Molecular and cellular biology 24, 8055-68. PMID: 15340068

2. Babu, JR. et al. (2005) Journal of neurochemistry 94, 192-203. PMID: 15953362

3. Bjørkøy, G. et al. (2005) The Journal of cell biology 171, 603-14. PMID: 16286508

4. Pankiv, S. et al. (2007) The Journal of biological chemistry 282, 24131-45. PMID: 17580304

5. Clausen, TH. et al. (2010) Autophagy 6, 330-44. PMID: 20168092

6. Matsumoto, G. et al. (2011) Molecular cell 44, 279-89. PMID: 22017874

7. Taillebourg, E. et al. (2012) Autophagy 8, 767-79. PMID: 22622177

8. Isakson, P. et al. (2013) Autophagy 9, 1955-64. PMID: 24128730

9. Liu, X. et al. (2017) The Journal of cell biology 216, 1301-1320. PMID: 28404643

10. Zaffagnini, G. et al. (2018) The EMBO journal 37. PMID: 29343546

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SQSTM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

References

Datasheet

Anti-SQSTM1/p62 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-SQSTM1/p62 Polyclonal Antibody (HC648014)

Overview

Images

References

Datasheet

SDS

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